Thus, the analytical equations developed to calculate the collision at a fixed measuring frequency can be extended to map the whole curve over time. The absorption rate of solute on an adsorptive surface is found to follow a power-law decay function due to an evolving concentration gradient near the surface along with the depletion of the bulk solute molecules on the surface, for example, in a self-assembled monolayer adsorption kinetics. It also surprisingly suggests that due to the self-mimetic fractal function of diffusion, the equation should be corrected by a factor of two. The 1D diffusion simulations clarify that the dependence of the solute-surface collision rate on the observation-time (measurement time resolution) is because of the multiple collisions of the same molecules over different time regions. The equations are then compared to the results of several numerical Monte Carlo simulations using a random walk model.

Here an analytical solution of Fick’s 2nd law is used to predict the diffusion and the stochastic adsorption of single diluted solute molecules on flat and patterned surfaces. At a low excitation laser power density, the photobleaching of YOYO-1 is too slow and a minimum required power density was identified, which can be theoretically predicted with the proposed method in this report. In this paper, we study the photobleaching of YOYO-1 and its correlation with the quality of the PAINT images. Thus, controlling the fluorescence on–off rate is important in PAINT.

Many YOYO-1 molecules intercalate into DNA and remain there during imaging, and most of them have to be temporarily or permanently fluorescently bleached, often stochastically, to allow for the visualization of a few fluorescent events per DNA per frame of the video. In a typical PAINT video acquisition, dye molecules (YOYO-1) in solution sparsely bind to the target surfaces (DNA) whose locations can be mathematically determined by fitting their fluorescent point spread function. Super-resolution imaging of single DNA molecules via point accumulation for imaging in nanoscale topography (PAINT) has great potential to visualize fine DNA structures with nanometer resolution.

Super yoyo batch